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A collection of articles covering the practical and theoretical considerations behind the use of capillary electrophoresis (CE) for the analysis of small oligonucleotides and modified nucleotides. Protocols offer time-tested advice on instrumentation, signal detection, the capillary environment and the integration of mass spectrometry with CE. Several chapters are devoted to the analysis of small therapeutic oligonucleotides, nucleosides, and ribonucleotides. Articles, by specialists in the field, are illustrated with tables and charts.

Book Info

Univ. of Queensland, Australia. Combines methods of mass spectrometry and capillary electrophoresis. Describes micro-device capillary electrophoresis methods. For basic and clinical researchers.

Preface v
Contents of Companion Volume xiii
Contributors xvii
Part I Analytical Considerations for Capillary Electrophoresis 1
1 Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis 3
2 DNA Separation Mechanisms During Electrophoresis 27
3 Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis 43
4 Quantitative Measurements 51
5 Microchip-Based Capillary Electrophoresis Systems 67
6 DNA Sequencing by Capillary Array Electrophoresis 85
7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis 95
Part II Capillary Matrix: Considerations for Capillary Electrophoresis 109
8 Influence of Polymer Concentration and Polymer Composition on Capillary Electrophoresis of DNA 111
9 Capillary Coatings: Choices for Capillary Electrophoresis of DNA 125
10 Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis 139
11 Sieving Matrix Selection 167
12 Parameters Affecting Capillary Electrophoretic Separation of DNA 189
Part III Electrophoresis Matrix: Applications for DNA Fragment Sizing 201
13 DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals 203
14 Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material 215
15 DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium 225
16 DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries 239
17 Capillary Electrophoresis with Glycerol as an Additive 259
18 Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels 279
Part IV Practical Applications for Large DNA Fragment Sizing and Fragment Collection 291
19 Robust Field Inversion Capillary Electrophoretic Separation of Long DNA Fragments 293
20 Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments 307
21 Collection of Capillary Electrophoresis Fractions on a Moving Membrane 323
22 Separation of Supercoiled DNA Using Capillary Electrophoresis 333
Part V Practical Applications for Oligonucleotide and Nucleotide Analysis 345
23 Quality Control of Nucleotides and Primers for PCR 347
24 Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis 353
25 Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides 371
26 Capillary Electrophoresis Separation of Ribonucleosides 377
27 On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Capillaries 383
Part VI Practical Applications for Analysis of Environmental Damage and Cellular Metabolism of DNA 393
28 Capillary Electrophoresis--Mass Spectrometric Analysis of DNA Adducts 395
29 Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis 407
30 Assay for DNA Damage Using Immunochemical Recognition and Capillary Electrophoresis 419
31 Analysis of DNA Damage Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry 429
32 Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Sieving Electrophoresis 443
33 Capillary Electrophoresis of Urinary Normal and Modified Nucleosides of Cancer Patients 459
Index 475