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Matrix Metalloproteinase Protocols |
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From Book News, Inc.
Details proven techniques for expression and purification of matrix metalloproteinases (MMPs) and TIMPs, and for the detection of MMPs and TIMPs at both protein and mRNA levels. Part I overviews the MMP arena, and Parts II and III present methods with step-by-step instructions and notes on variant applications and pitfalls. Part IV shows how to assay MMP and TIMP activities in a wide variety of circumstances. Of interest to those in chemistry, biology, and histology. Specific topics include strategies for cloning new MMPs and TIMPs, expression of recombinant membrane-type MMPs, and antibodies to MMP-cleaved aggrecan. Clark is affiliated with the University of East Anglia.
Book Info
Univ. of East Anglia, Norwich, UK. Comprehensive set of proven and cutting-edge methods for studying MMPs and TIMPs. Summarizes the experience of expert laboratorians, and presents reproducible techniques for the expression and purification of MMPs and TIMPs. For researchers.
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