Book Info

CABI Bioscience UK Centre, Surrey, UK. Presents the principles for gene probe design, labeling, detection, target format, and hybridization conditions together with detailed protocols, practical hints, and tips. For researchers. Outline format.

Book Description

CABI Bioscience UK Centre, Surrey, UK. Presents the principles for gene probe design, labeling, detection, target format, and hybridization conditions together with detailed protocols, practical hints, and tips. For researchers. Outline format.

Preface
Contributors
1 Target Format and Hybridization Conditions 1
2 End Labeling Procedures: An Overview 13
3 Photobiotin Labeling 19
4 Nick Translation 23
5 Random Primed Labeling 27
6 Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization 29
7 Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors 43
8 The [beta]-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites 61
9 Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma 85
10 Detection of Chlamydia trachomatis by DNA Amplification 93
11 Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes 101
12 Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction 113
13 Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes 137
14 Stable Isotope Probing for Detection of Methanotrophs After Enrichment with [superscript 13]CH[subscript 4] 149
15 Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes) 159
16 Analysis of 16S-23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species 171
17 Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes 179
18 In Situ Hybridization of Burkholderia cepacia Cells 187
19 Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil 193
20 Detection of Single-Copy Genes in DNA from Transgenic Plants 211
21 Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission 223
22 Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food 235
23 Polymerase Chain Reaction for Detection of Listeria monocytogenes 245
Index 259