From Book News, Inc.

Due to improved isolation techniques and discoveries allowing sufficient dendritic cells (DC) to be derived to study for immunotherapy applications, it is now known that these once enigmatic "veiled" cells are involved in complex immune roles. In 38 papers, international researchers describe techniques for: DC isolation from animal tissues; isolation and characterization of human DC (with a contribution by Robinson-hematology, U. College Hospital, London); derivation of animal DC from rat bone marrow; generation of human DC from progenitor cells; and analysis of aspects of DC function. Stagg (Antigen Presentation Research Group, Imperial College School of Medicine; Middlesex, UK) discusses the isolation of mouse spleen DC. A photo shows a lymph drainage system installed in a human volunteer.Book News, Inc.

Book Info

Univ. College Hospital, London, UK. Features state-of-the-art collection of laboratory techniques, with readily reproducible techniques detailed step-by-step. Also gives proven methods for isolating dendritic cells from a wide variety of tissues. DNLM: Dendritic Cells--physiology.

Book Description

Stephen P Robinson, MD, PhD, and Andrew Satgg, PhD, have brought together a wide range of time-proven methods for studying these dendritic cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide vareity of tissues, or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeueutics.

Foreword
Preface
Contributors
Pt. I Isolation of Dendritic Cells from Animal Tissues
1 Isolation of Dendritic Cells from Mouse Lymph Nodes 3
2 Isolation of Mouse Spleen Dendritic Cells 9
3 Isolation of Mouse Thymic Dendritic Cells 23
4 Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen 29
5 Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells 43
6 Murine Intestinal Dendritic Cell Isolation 63
7 Identification and Isolation of Rodent Lung Dendritic Cells 73
8 Isolation and Propagation of Mouse Liver-Derived Dendritic Cells 85
Pt. II Isolation and Characterization of Human Dendritic Cells
9 Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells 99
10 Purification of Dendritic Cells from Peripheral Blood 111
11 Isolation of Human Tonsillar Dendritic Cells 121
12 Isolation of Dendritic Cells from Human Afferent Lymph 137
13 Isolation of Human Skin Dendritic Cells by In Vitro Migration 145
14 Human Intestinal Dendritic Cell Isolation 155
15 Isolation of Human Lung Dendritic Cells 163
16 Identification and Isolation of Synovial Dendritic Cells 175
Pt. III Derivation of Animal Dendritic Cells from Progenitor Cells
17 Generation of Murine Bone-Marrow-Derived Dendritic Cells 191
18 Generation of Dendritic Cells from Rat Bone Marrow 199
19 Derivation of Murine Dendritic Cells from Thymic Precursors 207
Pt. IV Generation of Murine Dendritic Cell Lines
20 Generation of Mouse Dendritic Cells Lines 219
21 Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin 231
22 Derivation of Dendritic Cell Lines from Mouse Skin 243
Pt. V Generation of Human Dendritic Cells from Progenitor Cells
23 Propagation of Human Dendritic Cells In Vitro 257
24 Generation of Dendritic Cells from Lymphoid Precursors 275
25 Dendritic Cells Generation from Highly Purified CD14' Monocytes 283
26 Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy 297
Pt. VI Aspects of Dendritic Cell Function
27 Chemotaxis of In Vitro Cultured Human Dendritic Cells 307
28 Adhesion of Dendritic Cells to Endothelia 313
29 Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells 325
30 In Vivo Assays of Langerhans Cell Migration 331
31 In Vitro Migration of Rat Dendritic Cells 347
32 Antigen Uptake by Dendritic Cells 369
33 Uptake of Antigen-Antibody Complexes by Human Dendritic Cells 377
34 Immunoelectron Microscopy of Antigen Processing in Dendritic Cells 387
35 Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells 413
36 Detection of Intracellular Cytokines in Dendritic Cells 423
37 Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells 429
38 Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells 441
Index 455