From Book News, Inc.

Researchers in the field, including members of the Dolly team, present reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes attention to the creation of genetically modified murine stains. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional gene targeting. Techniques are detailed for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Clarke teaches in the School of Biosciences at Cardiff University, UK.Book News, Inc.

Book Info

Cardiff Univ., UK. Features cutting-edge protocols for generating and maintaining genetically modified murine stains and includes techniques for cloning, conditional transgenesis, and cryopreservation of germlines. Emphasis is on manipulation of the mammalian genome. Previous edition: c1993.