Book Info

Ottawa Health Research Institute, Canada. Features detailed protocols for establishing and characterizing various lineages using ES cells, reproducible protocols for the cellular and molecular manipulation of differentiating ES cells, techniques for use with ES cells, and tips from experts on avoiding pitfalls. Outline format.

Preface
Contributors
Color Plates
1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells 1
2 The Use of a Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos 17
3 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells 27
4 Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation 35
5 Effects of Altered Gene Expression on ES Cell Differentiation 45
6 Hypoxic Gene Regulation in Differentiating ES Cells 55
7 Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells 63
8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development 71
9 Analysis of Bcr-Abl Function Using an in Vitro Embryonic Stem Cell Differentiation System 83
10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development 97
11 Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells 107
12 Embryonic Stem Cell Differentiation and the Vascular Lineage 117
13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation 127
14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications 157
15 Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System 169
16 Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells 189
17 Lineage Selection for Generation and Amplification of Neural Precursor Cells 205
18 Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson's Disease 217
19 Epidermal Lineage 229
20 ES Cell Differentiation Into the Hair Follicle Lineage in Vitro 255
21 Embryonic Stem Cells as a Model for Studying Melanocyte Development 261
22 Using Progenitor Cells and Gene Chips to Define Genetic Pathways 269
23 ES Cell-Mediated Conditional Transgenesis 285
24 Switching on Lineage Tracers Using Site-Specific Recombination 309
25 From ES Cells to Mice: The Gene Trap Approach 335
26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells 347
27 Phage-Displayed Antibodies to Detect Cell Markers 381
28 Gene Transfer Using Targeted Filamentous Bacteriophage 393
29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors 403
30 Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays 417
31 Expression Profiling Using Quantitative Hybridization on Macroarrays 425
32 Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells 433
33 Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays 447
34 Direct Selection of cDNAs by Phage Display 461
35 Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System
Index 487