The manual has been written according to the MCI syllabus of practical biochemistry for first year MBBS students. All practicals are based on standard methods which can be easily reproduced. Complete details of practicals including preparation of reagents are given. Nineteen cases of Clinico-Biochemical History have been given for the benefit of the students. Clinical significance of each practical has been adequately explained. The manual can be used for observations, calculations and results, hence, there is no need for a separate practical notebook. It covers various aspects of biochemistry, including the role of clinical biochemistry in patient care.