Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.


　≫　Focus on 8-10 multicolor panels, allowing readers to effectively understand how to plan and apply the panels in the most constructive way
　≫　Features up-to-date information and references, compiled by experts in the field
　≫　Illustrations are made using multiple analysis software options, highlighting the key features of various diseases, to best inform on diagnostic features

List of contributors
Preface

1. Flow cytometry in clinical hematopathology: basic principles and data analysis of multiparameter data sets
　　-　Francis Lacombe and Marie C. Béné
2. Antigens
　　-　Marie-Christine Béné and Anna Porwit
3. Flow cytometry of normal blood, bone marrow and lymphatic tissue
　　-　Anna Porwit and Marie-Christine Béné
4. Reactive conditions and other diseases where flow cytometric findings may mimic hematological malignancies
　　-　Wolfgang Kern, Marie-Christine Béné and Anna Porwit
5. Examples of immunophenotypic features in various categories of acute leukaemia
　　-　Marie-Christine Béné and Anna Porwit
6. Acute lymphoid leukaemias and minimal residual disease in all
　　-　Giuseppe Basso, Barbara Buldini and Andrea Zangrando
7. Immunophenotyping of mature B-cell lymphomas
　　-　Olof Axler and Anna Porwit
8. Plasma cell myeloma and related disorders
　　-　Ruth M. de Tute, Andrew C. Rawstron and Roger G. Owen
9. Mature T-cell Neoplasms and NK-cell malignancies
　　-　Anne Tierens
10. Flow cytometric diagnosis of hodgkin lymphoma in lymph nodes
　　-　Lori Soma, Brent L. Wood and Jonathan R. Fromm
11. Minimal residual disease in acute myeloid leukaemia
　　-　Gerrit J. Schuurhuis, Angèle Kelder, Gert J. Ossenkoppele, Jacqueline Cloos and Wendelien Zeijlemaker
12. Ambiguous lineage and mixed phenotype acute leukemia
　　-　Anna Porwit and Marie-Christine Béné
13. Flow cytometry in myelodysplastic syndromes
　　-　T. M. Westers and A. A. van de Loosdrecht
14. Future applications of flow cytometry and related techniques
　　-　Marie Christine Béné and Francis Lacombe

Index