Practical Transfusion Medicine, Fourth Edition

Edited by

Michael F. Murphy, Professor of Blood Transfusion Medicine, University of Oxford; Consultant Haematologist, NHS Blood and Transplant and Oxford University Hospitals, Oxford, UK

Derwood H. Pamphilon, formerly Consultant Haematologist, NHS Blood and Transplant; Honorary Clinical Reader, Department of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

Nancy M. Heddle, Director, MTRP, Professor, Department of Medicine, McMaster University, Hamilton, Ontario, Canada



The pace of change in transfusion medicine is relentless, with new scientific and technological developments. In addition, efforts are continuing to improve clinical transfusion practice and avoid the use of blood wherever possible.

This fourth edition continues to be a concise and comprehensive guide to transfusion medicine. It has been thoroughly revised and updated throughout. The scope of the book has been broadened by including more international authors and a new co-editor, Professor Nancy Heddle, an expert in evidence-based transfusion medicine.

Practical Transfusion Medicine is divided into seven main parts, each of which guide the reader systematically through the key areas of the speciality:

- Principles of transfusion medicine

- Complications of transfusion

- Practice in blood centres and hospitals

- Clinical transfusion practice

- Alternatives to transfusion

- Cellular and tissue therapy and organ transplantation

- Development of the evidence base for transfusion

This book is ideal for trainee/resident haematologists revising for examinations and as a reference source for clinical and scientific staff working in the field of transfusion medicine.


Titles of related interest

Klein: Mollison’s Blood Transfusion in Clinical Medicine, 11e

ISBN 9780632064540 (forthcoming 2013)

De Vries: Hemovigilance: An Effective Tool for Improving Transfusion Safety

ISBN 9780470655276 (August 2012)

McCullough: Transfusion Medicine, 3e

ISBN 978144433705 (December 2011)

List of contributors, ix

Preface to the Fourth Edition, xvi

1 Introduction: recent evolution of transfusion medicine, 1
Paul M. Ness

Part One: Basic Principles of Immunohaematology

2 Essential immunology for transfusion medicine, 11
Jaap Jan Zwaginga & S. Marieke van Ham

3 Human blood group systems, 21
Geoff Daniels

4 Human leucocyte antigens, 31
Cristina V. Navarrete

5 Platelet and neutrophil antigens, 45
David L. Allen, Geoffrey F. Lucas & Michael F. Murphy

Part Two: Complications of Transfusions

6 Investigation of acute transfusion reactions, 65
Kathryn E. Webert & Nancy M. Heddle

7 Haemolytic transfusion reactions, 77
Edwin J. Massey, Robertson D. Davenport & Richard M. Kaufman

8 Febrile and allergic transfusion reactions, 89
Mark K. Fung & Nancy M. Heddle

9 Acute lung injury after transfusion, 97
Steven H. Kleinman & Ram Kakaiya

10 Purported adverse effects of transfusion-related immunomodulation and of the transfusion of ‘old blood’, 106
Eleftherios C. Vamvakas

11 Transfusion-associated graft-versus-host disease and microchimerism, 116
Beth H. Shaz, Richard O. Francis & Christopher D. Hillyer

12 Posttransfusion purpura, 127
Michael F. Murphy

13 Transfusion-transmitted infections, 132
Roger Y. Dodd & Susan L. Stramer

14 Bacterial contamination, 144
Sandra Ram´ýrez-Arcos & Mindy Goldman

15 Variant Creutzfeldt–Jakob disease, 152
Marc L. Turner

16 Emerging infections and transfusion safety, 161
Roger Y. Dodd

Part Three: Practice in Blood Centres and Hospitals

17 Regulatory aspects of blood transfusion, 171
William G. Murphy, Louis M. Katz & Peter Flanagan

18 The role of haemovigilance in transfusion safety, 181
James P. AuBuchon & Katharine A. Downes

19 Donors and blood collection, 190
Ellen McSweeney & William G. Murphy

20 Blood donation testing and the safety of the blood supply, 201
Richard Tedder, Simon J. Stanworth & Mindy Goldman

21 Production and storage of blood components, 209
Rebecca Cardigan & Stephen Thomas

22 Medicolegal aspects of transfusion practice, 224
Patricia E. Hewitt

23 Blood transfusion in hospitals, 230
Erica M. Wood, Mark H. Yazer & Michael F. Murphy

24 Blood transfusion in a global context, 250
David J. Roberts, Alan D. Kitchen, Stephen Field, Imelda Bates & Jean Pierre Allain

Part Four: Clinical Transfusion Practice

25 Inherited and acquired coagulation disorders, 263
Vickie McDonald & Samuel J. Machin

26 Massive blood loss, 276
Beverley J. Hunt & John R. Hess

27 Good blood management in acute haemorrhage and critical care, 284
Gavin J. Murphy, Nicola Curry, Nishith Patel & Timothy S. Walsh

28 Haematological disease, 300
Lise J. Estcourt, Simon J. Stanworth & Michael F. Murphy

29 Blood transfusion in the management of patients with haemoglobinopathies, 314
David C. Rees

30 Heparin-induced thrombocytopenia, 322
Andreas Greinacher & Theodore E. Warkentin

31 Immunodeficiency and immunoglobulin therapy, 335
Siraj A. Misbah

32 Fetal, neonatal and childhood transfusions, 347
Irene Roberts, Naomi Luban & Helen V. New

33 Recombinant proteins in diagnosis and therapy, 368
Marion Scott

Part Five: Alternatives to Transfusion

34 Principles of patient blood management, 379
Aryeh Shander & Lawrence Tim Goodnough

35 Autologous transfusion, 390
Dafydd Thomas, Biddy Ridler & John Thompson

36 Blood substitutes, 399
David J. Roberts & Chris V. Prowse

37 Pharmacological agents and recombinant activated factor VIIa, 410
Beverley J. Hunt & Simon J. Stanworth

Part Six: Cellular and Tissue Therapy and Organ Transplantation

38 Regulation and accreditation in cellular therapy, 421
Derwood H. Pamphilon & Zbigniew M. Szczepiorkowski

39 Stem cell collection and therapeutic apheresis, 433
Khaled El-Ghariani & Zbigniew M. Szczepiorkowski

40 Haemopoietic stem cell processing and storage, 443
Ronan Foley & Pamela O’Hoski

41 Haemopoietic stem cell transplantation, 455
I. Grant McQuaker & Ian M. Franklin

42 Cord blood transplantation, 464
Rachael Hough

43 Recent advances in clinical cellular immunotherapy, 473
Mark W. Lowdell & Emma Morris

44 Tissue banking, 480
Akila Chandrasekar, Paul Rooney & John Kearney

Part Seven: Development of the Evidence Base for Transfusion

45 Observational and interventional trials in transfusion medicine, 491
Alan Tinmouth, Dean Fergusson & Paul C. H´ebert

46 Getting the most out of the evidence for transfusion medicine, 505
Simon J. Stanworth, Susan J. Brunskill, Carolyn Doree, Sally Hopewell & Donald M. Arnold

47 Variation in transfusion practice and how to influence clinicians’ use of blood in hospitals, 516
Simon J. Stanworth, J.J. Francis, Michael F. Murphy & Alan T. Tinmouth

48 Scanning the future of transfusion medicine, 524
Dana V. Devine, Walter H. Dzik & Zbigniew M. Szczepiorkowski

Index, 537

Colour plate section can be found facing page 432