From Book News, Inc.
A handbook that gathers together the widely dispersed techniques and methods in current cellular and molecular biology. Each of the 21 chapters covers the principles behind the various methods and techniques, detailed step-by-step description of each protocol, and applications of protocols to different systems. Many chapters include a troubleshooting guide. Plastic comb-binding. Annotation copyright Book News, Inc. Portland, Or. --This text refers to the Hardcover edition.

Book Description
The second edition of this bestselling book helps researchers, teachers, and students properly understand and use the major techniques and methods employed in cellular and molecular biology today. Each chapter covers the principles behind the various methods and techniques, a detailed step-by-step description of each protocol, and applications of the protocol to different systems. In addition to presenting new protocols in all chapters, Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes in vitro transcription methods, analysis of DNA sequences, and new bioseparation techniques. Several new chapters cover cellular and subcellular localization of proteins, combinatorial chemistry, and data mining.

Book Info
University of Michigan, Ann Arbor. Handbook of tested and widely used techniques and methods in cellular and molecular biology research. Covers detailed procedures, applications, and underlying principles. Some chapters have troubleshooting guides. --This text refers to the Hardcover edition.

Isolation and Purification of DNA. Isolation and Purification of RNA. Extraction and Purification of Enzymes/Proteins. Preparation of Nucleic Acid Probes. Southern Blot Hybridization. Northern Blot Hybridization. Preparation of Monoclonal and
Polyclonal Antibodies Against Specific Proteins. Western Blot Hybridization. cDNA Libraries. Genomic DNA Libraries. DNA Sequencing and Analysis. Analysis of Gene Expression by Competitive PCR Technology. DNA Mutagenesis. DNA Footprinting and Gel
Retardation Assay. In Vitro Transcription and Translation of mRNAs. Inhibition of Gene Expression by Antisense DNA and RNA. Gene Transfer and Expression in Plants. Plant Cell and Tissue Culture. Microscopy: LM, SEM, TEM, Confocal and Applications.
Cellular and Subcellular Localization of Gene Expression and Protein Products. Bioseparation Techniques and Their Applications.