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¡Ø±³È¯ ¹× ¹ÝÇ°¾È³» :
- ±³È¯À̳ª ¹ÝÇ°½Ã »óÇ° ¼ö·ÉÈÄ 3ÀÏÀ̳» ±³È¯À̳ª ¹ÝÇ° Àǻ縦 ¾Ë·ÁÁֽðí ÀÏÁÖÀÏÀ̳»¿¡ ÀúÈñÂÊÀ¸·Î »óÇ°ÀÌ ÀÔ°íµÇ¾î¾ßÇÕ´Ï´Ù. ½ÃÀÏÀÌ °æ°úµÇ¸é ¹ÝÇ°ÀÌ ºÒ°¡ÇÕ´Ï´Ù. - º¯½É¿¡ ÀÇÇÑ ±³È¯À̳ª ¹ÝÇ°½Ã, ¹è¼Ûºñ´Â °í°´´Ô²²¼ ºÎ´ãÇϽøç, ¿À¹è¼ÛÀ̳ª »óÇ° ºÒ·®½Ã¿¡´Â ¹«·á ±³È¯ÀÌ °¡´ÉÇÕ´Ï´Ù. - ¹ÝÇ°½Ã¿¡´Â ¹ÝÇ°»óÇ° °Ë¼öÈÄ Ä«µå Ãë¼Ò¸¦ Çص帮°Å³ª ÅëÀå,Àû¸³±ÝµîÀ¸·Î ȯºÒ Á¶Ä¡ ÇØ µå¸³´Ï´Ù. |
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- Á¦ÁÖµµ¹× µµ¼»ê°£Áö¿ªÀº Åùèºñ°¡ Ãß°¡µÉ¼ö ÀÖ½À´Ï´Ù. |
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½ÇÇè½Ç¿¡¼ÀÇ ¾ÈÀü¼öÄ¢
Á¦ 1 Àå
¹ÚÅ׸®¾Æ ¹è¾ç°ú plasmid vector
½ÇÇè 1. ¹ÚÅ׸®¾Æ ¹è¾ç ¹èÁö 34
½ÇÇè 2. Pure¹ÚÅ׸®¾ÆÀÇ ºÐ¸® ¹× ¹è¾ç 43
½ÇÇè 3. ¼¼±Õ ¼öÀÇ ÃøÁ¤ 49
½ÇÇè 4. ¹ÚÅ׸®¾Æ ¼¼Æ÷ÀÇ ¼ºÀå ¹× ±ÕÁÖ(strain) º¸°ü¹ý 56
½ÇÇè 5. Á¢ÇÕ(conjugation) 63
½ÇÇè 6. ¹ÚÅ׸®¾Æ ¼¼Æ÷·ÎºÎÅÍ plasmid DNAÀÇ
ÃßÃâ°ú Á¤Á¦ 66
½ÇÇè 7. ÇÙ»ê°ú ´Ü¹éÁúÀÇ ³óµµ ÃøÁ¤ 75
½ÇÇè 8. Agarose gel electrophoresis 79
½ÇÇè 9. Agarose gel ·ÎºÎÅÍ fragmented DNAÀÇ recovery ¹× Á¤Á¦ 86
½ÇÇè 10. Plasmid vector¸¦ ÀÌ¿ëÇÑ subcloning 90
½ÇÇè 11. Recombinant Plasmid DNAÀÇ ÇüÁúÀüȯ 98
½ÇÇè 12. ÇüÁúÀüȯüÀÇ °íµî»ý¹°Ã¼·ÎÀÇ ÇüÁúÀüȯ ¹× ºÐ¼® 105
½ÇÇè 13. ¥â-galactosidase enzymeÀÇ induction 118
½ÇÇè 14. Recombinant plasmid DNAÀÇ Å½Áö¿Í Á¦ÇÑÈ¿¼Ò
Áöµµ ÀÛ¼º 121
Á¦ 2 Àå
DNA¿Í ¹ÙÀÌ¿ÀÅ×Å©³î·¯Áö
½ÇÇè 15. ÁøÇÙ»ý¹°·ÎºÎÅÍ Genome DNAÀÇ ÃßÃâ 129
½ÇÇè 16. ÁøÇÙ DNAÀÇ ºÎºÐÀûÀÎ Á¦ÇÑÈ¿¼Ò Àý´Ü 133
½ÇÇè 17. Southern Hybridization 135
½ÇÇè 18. In situ hybridization 141
½ÇÇè 19. ¹ýÀÇÇÐÀû Ãø¸é¿¡¼ÀÇ fingerprinting 148
½ÇÇè 20. ÁßÇÕÈ¿¼Ò¿¬¼â¹ÝÀÀ(PCR, Polymerase Chain Reaction) 157
½ÇÇè 21. UV¿¡ ÀÇÇÑ DNA ¼Õ»ó ¹× ±¤È¸º¹ 161
Á¦ 3 Àå
RNA
½ÇÇè 22. °íµî»ý¹° Á¶Á÷À¸·ÎºÎÅÍ total RNAÀÇ ÃßÃâ 164
½ÇÇè 23. mRNA(poly(A)+)ÀÇ Á¤Á¦ ¹× ºÐ¸® 172
½ÇÇè 24. Northern hybridization 176
½ÇÇè 25. RNAÀÇ Dot and slot hybridization 184
Á¦ 4 Àå
ProteinÀÇ Á¤Á¦¿Í Expression
½ÇÇè 26. Tyrosinase ´Ü¹éÁúÀÇ ÃßÃâ ¹× Á¤Á¦ 188
½ÇÇè 27. Tyrosinase È¿¼ÒÀÇ »ýÈÇÐÀû Ư¼º 195
½ÇÇè 28. ¹ÚÅ׸®¾Æ(E.coli)¿¡¼ÀÇ fusion protein(cloned genes) ÀÇ Á¦Á¶ 199
½ÇÇè 29. SDS-Polyacrylamide Gel electrophoresis 205
½ÇÇè 30. Western blotting 213
½ÇÇè 31. Translation of mRNA 222
½ÇÇè 32. ¸ñÇ¥ ´Ü¹éÁú¿¡ ´ëÇÑ Immunoprecipitation 227
½ÇÇè 33. È¿¼Ò¿¡ °áÇÕÇÑ ¸é¿ªÈíÂøÁ¦ ºÐ¼®¹ý- ELISA(Enzyme-Linked
ImmunoSorvent Assay)¹ÝÀÀ 233
Á¦ 5 Àå
Bacteriophage ¥ë¸¦ ÀÌ¿ëÇÑ molecular cloning
½ÇÇè 34. Bacteriophage ¥ë¿¡ ´ëÇÑ plaqueÀÇ ºÐ¸® 237
½ÇÇè 35. single plaqueÀ¸·ÎºÎÅÍ bacteriophage ¥ë stockÀÇ Á¦Á¶ 241
½ÇÇè 36. ¼Ò±Ô¸ð bacteriophage ¥ë¿¡ ´ëÇÑ liquid cultureÀÇ Á¦Á¶ 245
½ÇÇè 37. Bacteriophage ¥ë DNAÀÇ Á¦Á¶ 248
½ÇÇè 38. Bacteriophage ¥ë DNAÀÇ Á¦ÇÑÈ¿¼Ò¿¡ ÀÇÇÑ digestion 251
½ÇÇè 39. Bacteriophage ¥ë DNA armsÀÇ Á¤Á¦ 254
½ÇÇè 40. Bacteriophage ¥ë vectorÀÇ alkaline phosphatase
È¿¼Ò·ÎÀÇ Ã³¸® 258
½ÇÇè 41. Bacteriophage ¥ëÀÇ foreign DNA fragment¿ÍÀÇ ligation and packaging 261
ºÎ·Ï
ºÎ·Ï 1. ºÐÀÚ»ý¹°ÇÐ °ü·Ã¿ë¾×Á¦Á¶ 267
ºÎ·Ï 2. ºÐÀÚ»ý¹°ÇÐ ¹°ÁúÀÇ ´ÜÀ§µé 278
ºÎ·Ï 3. ¿ÏÃæ¿ë¾× 279
ºÎ·Ï 4. ¾Ð·ÂÀ» ¿Âµµ·Î ȯ»êÇÏ´Â ¹ý 282
ºÎ·Ï 5. Á¤»óÀÎÀÇ ¼Òº¯ ¼ººÐÇ¥ 283
ºÎ·Ï 6. À¯¿ëÇÑ web sites 284
ºÎ·Ï 7. »ý¸í°øÇÐ Á¤º¸ web sites 287
ã¾Æº¸±â 291 |
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µî·ÏµÈ ¼ÆòÀÌ ¾ø½À´Ï´Ù. |
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